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STEMCELL Technologies Inc stemspan animal component-free (acf) media
Stemspan Animal Component Free (Acf) Media, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stemspan-acf+(animal+component-free)+media/stemspan+animal+component+free++acf++media/pmc11572293-225-8-14
Average 90 stars, based on 1 article reviews
stemspan animal component-free (acf) media - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Cell Culture:

Article Title: Envelope-Specific Adaptive Immunity following Transplantation of Hematopoietic Stem Cells Modified with VSV-G Lentivirus
Article Snippet: CD34 + cells were cultured on recombinant fibronectin fragment (Takara Bio, Mountain View, CA, USA)-coated culture flasks at a density of 1 × 10 6 cells/mL and 2.9 × 10 5 cells/cm 2 in StemSpan animal component-free (ACF) media (STEMCELL Technologies, Vancouver, BC, Canada), supplemented with 4 μg/mL protamine sulfate (American Pharmaceutical Partners [APP], East Schaumburg, IL, USA), 100 ng/mL each of recombinant human stem cell factor (rhSCF), thrombopoietin (rhTPO), and Flt-3 ligand (rhFLT3L) (all from CellGenix, Freiburg, Germany), and 1 mM N -acetylcysteine (NAC) (Cumberland Pharmaceuticals, Nashville, TN, USA).

Article Title: Ex Vivo Expansion of CD34 + CD90 + CD49f + Hematopoietic Stem and Progenitor Cells from Non‐Enriched Umbilical Cord Blood with Azole Compounds
Article Snippet: UCB–MNC were cultured at an empirically determined optimal density of 4.0 × 10 5 cells/ml without any cell surface marker dependent stem cell enrichment in StemSpan‐Serum Free Expansion Media (SFEM) or StemSpan‐Animal Component Free Media (ACF) (STEMCELL Technologies, Canada) supplemented with human cytokine cocktail of 100 ng/ml stem cell factor (SCF) (PeproTech, USA) and thrombopoietin (TPO) (PeproTech); 50 ng/ml FLT‐3 Ligand (FLT‐3L) (PeproTech); and 20 ng/ml insulin‐like growth factor binding protein‐2 (IGFBP‐2) (R&D Systems, USA).

Recombinant:

Article Title: Envelope-Specific Adaptive Immunity following Transplantation of Hematopoietic Stem Cells Modified with VSV-G Lentivirus
Article Snippet: CD34 + cells were cultured on recombinant fibronectin fragment (Takara Bio, Mountain View, CA, USA)-coated culture flasks at a density of 1 × 10 6 cells/mL and 2.9 × 10 5 cells/cm 2 in StemSpan animal component-free (ACF) media (STEMCELL Technologies, Vancouver, BC, Canada), supplemented with 4 μg/mL protamine sulfate (American Pharmaceutical Partners [APP], East Schaumburg, IL, USA), 100 ng/mL each of recombinant human stem cell factor (rhSCF), thrombopoietin (rhTPO), and Flt-3 ligand (rhFLT3L) (all from CellGenix, Freiburg, Germany), and 1 mM N -acetylcysteine (NAC) (Cumberland Pharmaceuticals, Nashville, TN, USA).

Article Title: Ex Vivo Expansion of CD34 + CD90 + CD49f + Hematopoietic Stem and Progenitor Cells from Non‐Enriched Umbilical Cord Blood with Azole Compounds
Article Snippet: UCB–MNC were cultured at an empirically determined optimal density of 4.0 × 10 5 cells/ml without any cell surface marker dependent stem cell enrichment in StemSpan‐Serum Free Expansion Media (SFEM) or StemSpan‐Animal Component Free Media (ACF) (STEMCELL Technologies, Canada) supplemented with human cytokine cocktail of 100 ng/ml stem cell factor (SCF) (PeproTech, USA) and thrombopoietin (TPO) (PeproTech); 50 ng/ml FLT‐3 Ligand (FLT‐3L) (PeproTech); and 20 ng/ml insulin‐like growth factor binding protein‐2 (IGFBP‐2) (R&D Systems, USA).

Marker:

Article Title: Envelope-Specific Adaptive Immunity following Transplantation of Hematopoietic Stem Cells Modified with VSV-G Lentivirus
Article Snippet: CD34 + cells were cultured on recombinant fibronectin fragment (Takara Bio, Mountain View, CA, USA)-coated culture flasks at a density of 1 × 10 6 cells/mL and 2.9 × 10 5 cells/cm 2 in StemSpan animal component-free (ACF) media (STEMCELL Technologies, Vancouver, BC, Canada), supplemented with 4 μg/mL protamine sulfate (American Pharmaceutical Partners [APP], East Schaumburg, IL, USA), 100 ng/mL each of recombinant human stem cell factor (rhSCF), thrombopoietin (rhTPO), and Flt-3 ligand (rhFLT3L) (all from CellGenix, Freiburg, Germany), and 1 mM N -acetylcysteine (NAC) (Cumberland Pharmaceuticals, Nashville, TN, USA).

Article Title: Ex Vivo Expansion of CD34 + CD90 + CD49f + Hematopoietic Stem and Progenitor Cells from Non‐Enriched Umbilical Cord Blood with Azole Compounds
Article Snippet: UCB–MNC were cultured at an empirically determined optimal density of 4.0 × 10 5 cells/ml without any cell surface marker dependent stem cell enrichment in StemSpan‐Serum Free Expansion Media (SFEM) or StemSpan‐Animal Component Free Media (ACF) (STEMCELL Technologies, Canada) supplemented with human cytokine cocktail of 100 ng/ml stem cell factor (SCF) (PeproTech, USA) and thrombopoietin (TPO) (PeproTech); 50 ng/ml FLT‐3 Ligand (FLT‐3L) (PeproTech); and 20 ng/ml insulin‐like growth factor binding protein‐2 (IGFBP‐2) (R&D Systems, USA).

Binding Assay:

Article Title: Envelope-Specific Adaptive Immunity following Transplantation of Hematopoietic Stem Cells Modified with VSV-G Lentivirus
Article Snippet: CD34 + cells were cultured on recombinant fibronectin fragment (Takara Bio, Mountain View, CA, USA)-coated culture flasks at a density of 1 × 10 6 cells/mL and 2.9 × 10 5 cells/cm 2 in StemSpan animal component-free (ACF) media (STEMCELL Technologies, Vancouver, BC, Canada), supplemented with 4 μg/mL protamine sulfate (American Pharmaceutical Partners [APP], East Schaumburg, IL, USA), 100 ng/mL each of recombinant human stem cell factor (rhSCF), thrombopoietin (rhTPO), and Flt-3 ligand (rhFLT3L) (all from CellGenix, Freiburg, Germany), and 1 mM N -acetylcysteine (NAC) (Cumberland Pharmaceuticals, Nashville, TN, USA).

Article Title: Ex Vivo Expansion of CD34 + CD90 + CD49f + Hematopoietic Stem and Progenitor Cells from Non‐Enriched Umbilical Cord Blood with Azole Compounds
Article Snippet: UCB–MNC were cultured at an empirically determined optimal density of 4.0 × 10 5 cells/ml without any cell surface marker dependent stem cell enrichment in StemSpan‐Serum Free Expansion Media (SFEM) or StemSpan‐Animal Component Free Media (ACF) (STEMCELL Technologies, Canada) supplemented with human cytokine cocktail of 100 ng/ml stem cell factor (SCF) (PeproTech, USA) and thrombopoietin (TPO) (PeproTech); 50 ng/ml FLT‐3 Ligand (FLT‐3L) (PeproTech); and 20 ng/ml insulin‐like growth factor binding protein‐2 (IGFBP‐2) (R&D Systems, USA).



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